Exceptional Metachromatic Staining: Toluidine Blue O exhibits metachromasia, staining certain polyanionic structures (like mast cell granules, cartilage matrix, and mucins) purple to red-violet (metachromatically), while staining other structures a standard blue (orthochromatically). This unique property provides rich differential contrast.
Visualization of Specific Structures: Ideal for clearly demonstrating:
Mast Cells: Their granules stain intensely metachromatically, crucial for studying allergic reactions and inflammation.
Cartilage and Connective Tissue: The ground substance (proteoglycans) stains metachromatically.
Mucins: Identifies mucin-secreting cells and pathologies involving mucus.
Nissl Substance: Stains RNA-rich areas in neurons (Nissl bodies) a distinct blue, vital for neurohistology.
Bacteria and Fungi: Can be used as a general stain for microorganisms.
High Purity & Consistency: Manufactured to stringent histology-grade standards, ensuring minimal impurities and a consistent dye concentration. This guarantees reproducible and reliable staining performance batch after batch.
Versatile Application: Used across a broad spectrum of disciplines, including:
Pathology: For rapid diagnosis of biopsy specimens (e.g., assessing margins).
Neuroscience: For visualizing neurons and glial cells.
Connective Tissue Research: To study mast cell distribution and ground substance.
Microbiology: As a general stain for cells and bacteria.
Reliable Performance: Every lot undergoes rigorous quality control to ensure consistent staining intensity and dependable results, providing confidence in your diagnostic and research outcomes.
Histology
TOLUIDINE BLUE O, 1% AQ
VION Biosciences
Uncover intricate cellular details and differentiate various tissue components with VION Histology Grade Toluidine Blue O. This versatile basic thiazine dye is renowned for its metachromatic properties, allowing it to stain different tissue elements in distinct colors, making it invaluable for both routine and specialized histological and cytological applications.
$0.00
Histology Grade
Melting Point ~0°C (32°F in water)
Boiling Point ~100°C (212°F in water)
CAS Min % 1%, 99%
CAS Max % 99%, 1%
Concentration 1%
Dye Content 1%
Assay Percent Range 1%
Extinction Coefficient 13000 M-1 cm-1
UN Number Not regulated.
Solubility Information Complete in water.
Identification Toluidine Blue O, 1% Aqueous
Infrared Spectrum Yes
Lambda Max 241-247 nm
Percent Purity 1%
Grade Data not available.
Concentration or Composition (by Analyte or Components) 1% Aqueous
Molar Absorption Coefficient 13000 M-1 cm-1
Odor No odor.
Packaging HDPE Bottle
Specific Gravity 1.0 g/mL in water)
Vapor Pressure 14 mm Hg in water)
Certificate of Analysis Yes
Decomposition Information Data not available.
Density 1.0 g/mL in water)
pH Color Changes Data not available.
Solvent or Matrix Water
Color Blue
pH Data not available.
Quantity 500 mL
Physical Form Data not available.
Chemical Name or Material Toluidine Blue O
CAS 92-31-9, 7732-18-5
Molecular Formula C15H16ClN3S
Molecular Weight (g/mol) 305.82
MDL Number MFCD00011934
InChI Key HNONEKILPDHFOL-UHFFFAOYSA-M
Synonym Toluidine Blue O, Water Solution
PubChem CID Data not available.
IUPAC Name 3-amino-7-(dimethylamino)-2-methyl-5λ⁴-phenothiazin-5-ylium chloride
SMILES [Cl-].CN(C)C1=CC=C2N=C3C=C(C)C(N)=CC3=[S+]C2=C1
Contact your preferred distribution channel or VION directly
Custom sizes available upon request
