Vital Stain for Live Cells: Neutral Red selectively accumulates in the lysosomes of living cells, staining them a distinct red. This property makes it an excellent indicator of cell viability and membrane integrity, as only cells with intact lysosomal membranes can retain the dye.
Visualization of Acidic Organelles: Beyond live cell assays, in fixed tissues, Neutral Red effectively stains lysosomes and other acidic components (like Nissl substance in neurons), providing valuable morphological detail.
Counter stain for Nuclear Stains: It can serve as a valuable counter stain in some protocols, offering a contrasting reddish hue to highlight cytoplasmic features when used with a nuclear stain.
High Purity for Reliable Results: Manufactured to stringent histology-grade standards, ensuring minimal impurities that could interfere with specific cellular uptake or lead to non-specific staining. This guarantees clearer and more accurate observations.
Consistent Performance: Each batch undergoes rigorous quality control to guarantee consistent staining intensity and dependability, crucial for reproducible results in both qualitative and quantitative assays.
Versatile Applications:
Cell Viability and Cytotoxicity Assays: A common method for assessing the health and survival of cell cultures.
Lysosomal Studies: For visualizing and studying the distribution and function of lysosomes.
Histology and Cytology: As a component in various staining protocols to highlight specific cellular features.
Histology
NEUTRAL RED, 1% AQ
VION Biosciences
Illuminate cell viability and visualize acidic cellular components with VION Histology Grade Neutral Red. This versatile and widely used stain is essential for live cell studies, viability assays, and the clear identification of lysosomes and other acidic organelles within fixed tissue.
$0.00
Histology Grade
Melting Point 0°C
Boiling Point 100°C
CAS Min % 1%, 99%
CAS Max % 99%, 1%
Concentration 1%
Dye Content 1%
Assay Percent Range 1%
Extinction Coefficient 171004 M-1cm-1
Linear Formula C15H17N4
UN Number Not regulated.
Solubility Information Complete in water.
Infrared Spectrum Yes
Lambda Max 540 – 543 nm
Percent Purity 1%
Grade Data not available.
Concentration or Composition (by Analyte or Components) 1% in Water
Molar Absorption Coefficient 171004 M-1cm-1
Odor Mild characteristic odor.
Packaging HDPE Bottle
Specific Gravity 1.0 g/mL in water)
Vapor Pressure 14 mm Hg in water)
Certificate of Analysis Yes
Decomposition Information Data not available.
Density 1.0 g/mL in water)
pH Color Changes Data not available.
Solvent or Matrix Water
Color Red
pH Data not available.
Quantity 500 mL
Flash Point Data not available.
Physical Form Data not available.
Chemical Name or Material Neutral Red
CAS 553-24-2, 7732-18-5
Molecular Formula C15H17ClN4
Molecular Weight (g/mol) 288.78
MDL Number MFCD00012651
InChI Key PGSADBUBUOPOJS-UHFFFAOYSA-N
Synonym Neutral Red, Water Solution
PubChem CID Data not available.
ChEBI CHEBI:86370
IUPAC Name hydrogen N2,N2,7-trimethylphenazine-2,8-diamine chloride
SMILES [H+].[Cl-].CN(C)C1=CC=C2N=C3C=C(C)C(N)=CC3=NC2=C1
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Custom sizes available upon request
